Calpain-1 Potentiates Periodontal Regeneration via PHLPP1-ERK-Driven Osteogenesis in Periodontal Ligament Stem Cells.

ABSTRACT:

Objectives

In recent years, human periodontal ligament stem cells (hPDLSCs) are considered a promising seed cell source for bone tissue engineering, offering a prospective treatment for periodontal bone defects. The osteogenic differentiation of hPDLSCs is a critical process in bone tissue engineering. Calcium signalling regulates a myriad of cellular processes, including osteogenic differentiation; however, the function of calpain-1 (CAPN1), a calcium-dependent protein, remains incompletely elucidated.

Methods

Intracellular Ca²⁺ levels were evaluated using Fluo-4 AM assay. CAPN1 was overexpressed by transfection of lentivirus. Knockdown of CAPN1 was performed using siRNA transfection. mRNA and protein expression were detected through Real-time PCR and Western blo

SUBMITTER: Li Y 

PROVIDER: S-EPMC12723381 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature