Axonal structure-function relationships across experimental modalities.

ABSTRACT: The structure-function relationship of myelinated nerve fibers (Waxman & Bennett, 1972) links axon diameter and myelin thickness, commonly expressed as the g-ratio, to conduction velocity via saltatory mechanisms. Here, we investigated this relationship in the transcallosal motor pathway of the rat brain by combining functional and structural metrics in the same animals. Transcallosal conduction times (TCTs) were measured using local field potentials (LFPs) evoked by optogenetic stimulation of excitatory neurons in the motor cortex. Conduction velocity estimates were obtained by combining TCTs with transcallosal tract lengths derived from diffusion MRI (dMRI)-based tractography. Fluorescent labeling of the viral optogenetic construct verified the tractography trajectories. In parallel, axo

SUBMITTER: Skoven CS 

PROVIDER: S-EPMC12723409 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature