The <i>in vitro</i> stimulatory activity of CwlM∼P on mycobacterial MurA depends on a charged predicted interface that is distal from the CwlM phosphorylation site.

ABSTRACT: Mycobacterium tuberculosis and other mycobacteria have a thick cell wall that is tightly regulated - ensuring cell integrity and promoting antibiotic tolerance. The peptidoglycan layer of the cell wall is controlled at multiple points along the synthesis pathway. Here we focus on the stimulation of the peptidoglycan precursor enzyme MurA by its regulator CwlM. Using AlphaFold2 Multimer predictions we predicted a charged regulatory interface between CwlM and MurA that is separate from the phosphorylation site on CwlM that was previously shown to be required for activation. Using mutants of CwlM, we found that all of CwlM∼P's stimulation of MurA activity is dependent on this charged interface in vitro. However, our in vitro results here and previously suggest that MurA m

SUBMITTER: Hunt-Serracin AC 

PROVIDER: S-EPMC12723410 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature