Mechanistic Characterisation of Bacterial Terpene Synthases from Chitinophagaceae Producing Marine-Type Diterpenes.

ABSTRACT: Two neodolabellane-type diterpene synthases from Chitinophaga japonensis (CjCsS and CjNdtS) in addition to one enzyme from Dinghuibacter silviterrae (DsJS) were investigated. CjCsS produces the instable diterpene alcohol chitinosphaerol, which degrades upon chromatographic purification on silica gel to known presphaerol and isosphaerodienes I and II. Despite seemingly solid evidence in the literature, for all three compounds a structural revision is presented. Treatment of chitinosphaerol with N-bromosuccinimide resulted in the formation of the stable compounds bromosphaerols A and B. Phylogenetically unrelated CjNdtS catalyses the formation of neodolabella-1(14),2,7-triene, a compound that undergoes a remarkable thermal three-step sequence by Diels-Alder reaction to thermocyclene A, follo

SUBMITTER: Li H 

PROVIDER: S-EPMC12723471 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature