Genetic Optimization of Early 2009 Pandemic H1N1 Vaccine Strains for Improved Replication in Embryonated Chicken Eggs.

ABSTRACT: The 2009 pandemic H1N1 (pdm09) virus is both zoonotic and reverse-zoonotic, transmitting from swine to humans and vice versa. During the early zoonotic phase, immediately after the species jump and before substantial antigenic drift had accumulated, recombinant vaccine strains bearing hemagglutinin (HA) and neuraminidase (NA) from early pdm09 viruses often replicated poorly in embryonated chicken eggs (ECEs), contributing to delays and shortages in vaccine supply. Developing seed strains that are more productive in ECEs while preserving antigenicity and minimizing mammalian pathogenic potential is therefore essential for future pandemic preparedness. Efficient egg replication requires a balanced activity between HA and NA and their coordinated interaction with the polymerase subunit PB2. T

SUBMITTER: Son SE 

PROVIDER: S-EPMC12723478 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature