A Luminescence-Based Screening Platform for Lanthanide-Binding Peptides and Proteins.

ABSTRACT: The specific incorporation of lanthanide ions is a promising strategy to equip biomolecules with a new function. Their long-lived luminescence, strong anomalous X-ray scattering, paramagnetism, Lewis acidity, and photoredox activity are attractive features for protein-based probes, materials, and catalysts. However, natural lanthanide-binding proteins are rare, and de novo design is often complicated by unspecific binding to negatively charged patches on protein surfaces. We thus aimed to develop an efficient workflow to screen libraries of protein scaffolds for their ability to coordinate lanthanides. Here, we introduce a microtiter plate-based assay, which employs commercial filter plates and a dual readout based on sensitized Tb³⁺ luminescence. We first benchmarked our proced

SUBMITTER: Klassen R 

PROVIDER: S-EPMC12723673 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature