Pepsin Digestion for Proteomic Studies of the Human Hair Shaft.

ABSTRACT:

Rationale

Human hair shafts have received increased research interest owing to their easy accessibility and potential as a window for human health. Because the most abundant component in hair is protein, proteomics is a promising tool for studying the molecular composition of hair shafts. As one of the most sophisticated biomaterials, hair shafts also possess unique structures, particularly keratin intermediate filaments, posing challenges for proteomic sample processing. Previously, we discovered that incomplete trypsin proteolysis increased keratin sequence coverage but resulted in an abnormal stoichiometry between types I and II cuticular keratins (PMCID: 12130615).

Methods

In the present study, we explored the potential to re-examine the human hair proteome through pepsi

SUBMITTER: Yee DH 

PROVIDER: S-EPMC12723731 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature