Molecular mechanism of RBM15-mediated m6A modification in hepatitis B virus replication.

ABSTRACT:

Background

Hepatitis B virus (HBV) is a major human pathogen and chronically infects over 250 million people globally. The objective of our study is to investigate the mechanism of RBM15 in HBV replication, providing novel targets for HB treatment.

Methods

Huh-7 cells were treated with pHBV1.3. pHBV1.3 replication in Huh-7 cells was verified by detection of HBV RNAs, HBV pgRNA and HBx levels. The expression of RBM15, HULC and BRD4 was detected by qRT-PCR or WB. After RBM15 intervention, the effect of RBM15 on HBV replication was evaluated by detections of HBV DNA and HBV RNAs via q-PCR or qRT-PCR, HBsAg and HBeAg through ELISA. Total m6A levels were analyzed by m6A quantification. The m6A enrichment on HULC was analyzed by MeRIP. Bindings of HULC to ELAVL1, and ELAVL1 to BRD

SUBMITTER: Ni M 

PROVIDER: S-EPMC12723894 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature