Comparative assessment of SNP genotyping assays for challenging forensic samples utilizing ancient DNA methods.

ABSTRACT:

Background

The fields of ancient DNA research and forensic genetics share both methodological similarities and common challenges, particularly in the analysis of degraded DNA. Leveraging these overlaps, this study evaluates three single nucleotide polymorphisms (SNP)-based genotyping assays for analyzing challenging forensic samples: the FORCE-QIAseq SNP panel, the Twist ancient DNA hybridization capture panel, and whole-genome sequencing.

Results

We analyze twenty skeletal bone and tooth samples from authentic missing person cases, where almost all samples are severely degraded and contain exceptionally low amounts of endogenous DNA, reflected by both reduced quantifiable DNA concentrations and lower proportions of human DNA reads than typically obtained from high-quality f

SUBMITTER: Staadig A 

PROVIDER: S-EPMC12723910 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature