Effect of extracellular vesicle ZNF280B derived from lung cancer stem cells on lung cancer progression.

ABSTRACT:

Objective

The purpose of this research was to investigate the role of extracellular vesicles derived from lung cancer stem cells (lung CSCs-EVs) in lung cancer and to explore their potential mechanisms.

Methods

Lung CSCs were first isolated and verified using flow cytometry and RT-qPCR assays. Lung CSCs-EVs were extracted through ultracentrifugation and further characterized using transmission electron microscopy and Western blotting. The interaction between lung CSCs-EVs and lung cancer cells was observed through PKH67 staining. Subsequently, we analyzed the differentially expressed genes in lung CSCs using bioinformatics data analysis and evaluated the prognostic value of ZNF280B in lung cancer with the Kaplan-Meier Plotter. RT-qPCR was utilized to assess the mRNA expressi

SUBMITTER: Guo Q 

PROVIDER: S-EPMC12724098 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature