Characterization of HERV-K (HML-2) Rec proteins encoded in the human genome and their post-transcriptional function.

ABSTRACT: Human endogenous retrovirus K (HERV-K) proviruses of the HML-2 subgroup are the most recently integrated retroviral elements within the human genome. The HERV-K Rec protein, a functional homolog of HIV Rev, is essential for the expression of viral mRNAs with retained introns. However, their diversity and functional capacities have remained largely unexplored. We identified Rec coding sequences in the human genome and selected intact Rec proteins from 28 proviral loci for functional characterization. Using a dual-color fluorescent reporter containing the HERV-K Rec-response element and a complementary enzyme-linked immunosorbent assay, we found that Rec proteins from nine proviral genomic loci promoted function at the post-transcriptional level. All but one of these proviruses are insertion

SUBMITTER: Zurowska K 

PROVIDER: S-EPMC12724133 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature