A novel bispecific nanobody protects mice against RSV infection via intranasal administration.

ABSTRACT: Respiratory syncytial virus (RSV) is the leading cause of respiratory infection-related hospitalizations in children younger than 5 years. Neutralizing nanobody-based interventions represent a promising strategy against RSV. Here, we identify a novel nanobody (4-H1) targeting the RSV prefusion F (pre-F) protein, which demonstrates potent neutralization against both RSV A and B subtypes. Epitope characterization via binning assays, molecular docking, and mutational analyses revealed that 4-H1 interacts with a unique region within antigenic site Ø by engaging critical residues L207, K209, and the K65-N67-C69 cluster. To improve the in vivo efficacy and stability of the 4-H1 nanobody, we engineered a heterotrimeric bispecific nanobody (4-H1-anti-HSA-4-H1). This single-chain molecule co

SUBMITTER: Zhang M 

PROVIDER: S-EPMC12724135 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature