Real-time simultaneous monitoring of multiple analytes in bacterial cultures.

ABSTRACT: Bacterial metabolites are essential for biological processes, influencing human health, ecosystems, and various industrial applications. Simultaneous real-time monitoring of these metabolites is critical in understanding microbial dynamics, particularly in bioreactors and food or drug manufacturing. Current approaches often rely on offline methods, which are labor-intensive and susceptible to contamination, or genetic engineering techniques limited to single-analyte monitoring. Here, we present a novel method utilizing engineered periplasmic binding proteins (PBPs) conjugated with fluorophores to track multiple metabolites simultaneously in bacterial cultures, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, and <

SUBMITTER: Fink MM 

PROVIDER: S-EPMC12724168 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature