Multiple rotavirus species encode fusion-associated small transmembrane (FAST) proteins with cell type-specific activity.

ABSTRACT: Fusion-associated small transmembrane (FAST) proteins are viral nonstructural proteins that mediate cell-cell fusion to form multinucleated syncytia. We previously reported that human species B rotavirus NSP1-1 is a FAST protein that induces syncytia in primate epithelial cells but not rodent fibroblasts. We hypothesized that NSP1-1 proteins of other rotavirus species could mediate cell-cell fusion but might be limited to cell types derived from homologous hosts. To test this hypothesis, we predicted the structure and domain organization of NSP1-1 of species B rotavirus from a human, goat, and pig, species G rotavirus from a pigeon and turkey, and species I rotavirus from a dog and cat. We transiently expressed the NSP1-1 proteins in avian, canine, hamster, human, porcine, and simian cells

SUBMITTER: Sartalamacchia K 

PROVIDER: S-EPMC12724187 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature