A Gifsy prophage-encoded protein confers broad phage resistance in <i>Salmonella enterica</i> and is widely distributed across <i>Enterobacteriaceae</i>.

ABSTRACT: Phage Felix O1 (FO1) is one of the most widely used bacteriophages for targeting Salmonella enterica and is applied in both research and food safety settings. Despite its broad host range, we observed significantly reduced efficiency of plating (EOP) on a subset of Salmonella enterica strains, including serovar Typhimurium 4/74 (S. 4/74). We found that O-antigen-mediated steric hindrance contributes to this resistance, which can be partially overcome by supplementation with the SP6 phage tailspike protein (TSP). However, full restoration of the EOP required deletion of the Gifsy-1 prophage, leading to the identification of a single prophage-encoded defense gene, gipd474 (Gifsy-encoded phage defense protein from strain S. 4/74). Heterologous expression of

SUBMITTER: Bosma JS 

PROVIDER: S-EPMC12724215 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature