Metabolic and plasmid engineering to produce D-phenyllactic acid from glucose-xylose co-substrates in <i>Escherichia coli</i>.

ABSTRACT: Plasmid DNA comprises various modules, including promoter and terminator regions, replication origins, and antibiotic resistance genes as selection markers. The 5'UTRs of the promoter and ribosome binding site (RBS) have been extensively studied, and multiple synthetic promoters and RBSs have been developed. In this study, we focused primarily on the components of plasmid vectors in Escherichia coli, excluding the 5'UTRs. Six replication origins and four selection markers were shuffled, resulting in the construction of 24 different plasmids. The gene encoding D-lactate dehydrogenase from Cupriavidus necator was cloned into each plasmid in this plasmid library, and the resulting 24 plasmids were evaluated based on D-phenyllactic acid production. The maximum concentration and y

SUBMITTER: Noda S 

PROVIDER: S-EPMC12724218 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature