Genetic identification of the selenate reductase in <i>Enterobacter cloacae</i> SLD1a-1.

ABSTRACT: Bacterial selenate reduction is a key microbial process that affects the speciation and mobility of selenium in the environment. In this study, we identified the selenate reductase gene in the facultative anaerobe Enterobacter cloacae SLD1a-1. Genome sequencing revealed a membrane-bound, twin-arginine translocation (TAT) exported molybdoenzyme operon designated as srnABCD, under the regulation of the fumarate and nitrate reductase regulator (FNR) transcription factor. The srnA gene encodes a molybdenum-containing subunit; srnB and srnC encode iron-sulfur and membrane anchor subunits, respectively; and srnD encodes a TAT chaperone. Targeted mutagenesis of the srnA gene resulted in a mutant defective in selenate reduction. Complementation with

SUBMITTER: Phan J 

PROVIDER: S-EPMC12724226 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature