Development of new <i>Thermus thermophilus-Escherichia coli</i> shuttle vectors.

ABSTRACT: Genomic analysis revealed numerous plasmids in Thermus thermophilus strains isolated from the Senami Hot Spring in Japan. Five plasmids contained putative replication proteins (REP proteins) distinct from the pTT8-type commonly used in T. thermophilus-Escherichia coli shuttle vectors. Among them, two plasmids contained toxin-antitoxin-like tandemly aligned short open reading frames (ORFs) downstream of the putative REP protein. A series of shuttle vectors, pIOK, was constructed by cloning the putative REP protein gene and its flanking regions into an E. coli plasmid (ColE1, hygromycin-resistant). All five vectors were stably maintained in T. thermophilus HB27 in the presence of hygromycin. Among them, two containing the toxin-antitoxin-like module demonstrated g

SUBMITTER: Murayama S 

PROVIDER: S-EPMC12724237 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature