Imaging flow cytometry enables label-free cell sorting of morphological variants from populations of the unculturable bacterium <i>Pasteuria ramosa</i>.

ABSTRACT: Bacterial populations often display remarkable morphological heterogeneity. Fluorescence-activated cell sorting (FACS) is an important tool for understanding this diversity. FACs allows researchers to obtain pure samples of each morphological variant (or morphotype) that is present within a mixed population of cells and thus permits each morphotype to be phenotyped. In FACS, cells are first labeled with fluorescent markers, such as antibodies or transgenic constructs, and then separated out based on their possession of these labels. However, since the development of fluorescent labels requires a priori knowledge of bacterial biology, it is often impossible to apply FACS to understudied and/or unculturable bacteria. This challenge has limited our capacity to investigate the biology o

SUBMITTER: Vocelle D 

PROVIDER: S-EPMC12724247 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature