Investigating the antimethanogenic effects of selected nitro-compounds on methane production, rumen fermentation, and methanogenic archaea <i>in vitro</i>.

ABSTRACT: Enteric methane emissions (EME) cause adverse environmental effects and energy losses to the host. Given the potent antimethanogenic effects of the nitro compounds (NC) ethyl-nitroacetate (ENA), ethyl-2-nitropropionate (ENP), and nitrate (NO₃⁻), we hypothesized that they could effectively mitigate EME. Phase 1 determined dose responses of each NC, identified optimal doses, and evaluated their effects on gaseous composition, rumen fermentation, and bacterial-archaea populations. ENA and ENP tested at 8 and 16 mM inhibited CH₄ production by 100%, whereas NO₃⁻ tested at 12 and 24 mM reduced CH₄ production by 72%. However, the amount of spared H₂ differed among NC, revealing differential effects on fermentation pathways. ENA increased propionate and butyrate co

SUBMITTER: Castaneda A 

PROVIDER: S-EPMC12724261 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature