Characterization of the initial degradation mechanism involved in <i>C</i>-glycosylated isoflavone puerarin in soil microorganism.

ABSTRACT: Puerarin is C8-glycosylated daidzein and an active ingredient in the Chinese herbal medicine "Kakkonto." It is used in the early stage of cold treatment. A previous study reported that intestinal bacteria can remove the sugar moiety from puerarin. Despite their potential roles, C-glycosylated flavonoid-metabolizing microorganisms, including those acting on puerarin, have only been found in the gut but not been identified in natural environments. In this study, we identified a puerarin-catabolizing microorganism by screening from soil and clarified two-step deglycosylation reaction. The first step in the metabolism of puerarin was the oxidation of a sugar moiety, catalyzed by a FAD-dependent oxidase named PurA. PurA is the first example of a C-glycoside oxidase that showed act

SUBMITTER: Kumano T 

PROVIDER: S-EPMC12724307 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature