Comparative ubiquitinome analysis reveals the roles of protein ubiquitination in the heat stress response from <i>Metarhizium robertsii</i>.

ABSTRACT: Protein ubiquitination plays a vital role in the stress response of diverse filamentous fungi. However, few reports are available on fungal insect pathogens, including Metarhizium. Here, we report a comparative ubiquitylome analysis of Metarhizium robertsii exposed to heat stress. The growth of M. robertsii was suppressed, and protein ubiquitination levels were markedly promoted during heat stress. Compared to the control treatment, there were 4,674 sites with differential ubiquitination, of which 3,419 lysine ubiquitination sites across 1,344 proteins were significantly upregulated, and 1,255 sites on 750 proteins were downregulated under heat stress. Further analysis showed that these proteins with upregulated modified sites were preferentially enriched in the phenyl

SUBMITTER: Song J 

PROVIDER: S-EPMC12724308 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature