Resistance of endothelial cells to SARS-CoV-2 infection <i>in vitro</i>.

ABSTRACT: The secondary thrombotic/vascular clinical syndrome of COVID-19 suggests that SARS-CoV-2 infects the endothelium; however, robust in vitro infection of endothelial cells by various strains of SARS-CoV-2 remains to be demonstrated and continues to be debated. Here, we revisit the question of endothelial cell permissiveness to SARS-CoV-2 using isolated endothelial cells (from the lung, aorta, and endothelial cell progenitors), and additionally, to overcome limitations associated with cultured cells, using native endothelial cells within living precision cut human lung slices and single-cell RNA sequencing to track viral presence. Cellular infection in endothelial monocultures was determined using fluorescence imaging. Mediator release was measured by ELISA, and gene expression was ass

SUBMITTER: Ahmetaj-Shala B 

PROVIDER: S-EPMC12724323 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature