Sphingolipid-mediated vesiculation in multidrug-resistant <i>Sphingobacterium detergens</i> under polymyxin B stress.

ABSTRACT: Among environmentally widespread Sphingobacterium species, the animal feces isolate Sphingobacterium detergens E70 exhibited multidrug antibiotic resistance despite carrying few annotated antibiotic resistance genes (ARGs), prompting investigation of non-canonical mechanisms. This observation aligns with comparative genomic analysis of 62 Sphingobacterium genomes, which revealed only two to four ARGs per strain, typically associated with macrolide, phenicol, or tetracycline resistance. Remarkably, strain E70 lacked canonical ARGs yet displayed high-level resistance across nine antibiotics, including β-lactams and polymyxins. Scanning and transmission electron microscopy identified polymyxin B (PMB) as the most potent envelope-disrupting agent, revealing extensive outer

SUBMITTER: Min J 

PROVIDER: S-EPMC12724326 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature