Tracking poliovirus through wastewater: environmental surveillance insights from Haiti (2020-2023).

ABSTRACT: Environmental surveillance (ES) for poliovirus is a critical component of global eradication efforts, enabling the detection of virus circulation in communities, even in the absence of acute flaccid paralysis (AFP) cases. To monitor poliovirus circulation in a high-risk setting, ES was established in 2016 in Haïti in two cities (Port-au-Prince and Gonaïves) and expanded to five cities, adding Saint Marc, Cap Haïtien, and Port-de-Paix by 2023. Wastewater samples were collected monthly from 13 sites in urban areas and processed at CDC-Atlanta using the concentration and filtration elution (CaFÉ) method developed to concentrate enteroviruses, including polioviruses. Virus isolation was performed using poliovirus-sensitive (L20B) cells and enterovirus-sensitive (RD) cells, followed by molecula

SUBMITTER: Belgasmi-Allen H 

PROVIDER: S-EPMC12724337 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature