Proteolytic and non-proteolytic mechanisms of keratin degradation in <i>Onygena corvina</i> revealed by a proteogenomic approach.

ABSTRACT: Keratin-rich by-products from the poultry, textile, and leather industries pose a significant challenge for sustainable waste management due to their highly recalcitrant nature. While microbial degradation of keratin has been studied and may offer a viable solution, the complex enzyme machineries that are potentially needed to degrade these recalcitrant by-products remain partly unknown. In this study, we employed a high-resolution proteogenomic approach to characterize the keratinolytic machinery of Onygena corvina, a non-pathogenic saprophytic fungus. Using a membrane agar plate method with insoluble substrates, we obtained secretomes enriched in secreted and substrate-bound proteins during growth on α- and β-keratin-rich substrates, specifically wool and feather meal. Our finding

SUBMITTER: Pavale S 

PROVIDER: S-EPMC12724343 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature