Broad-spectrum inhibition of influenza A virus replication by blocking the nuclear export of viral ribonucleoprotein complexes.

ABSTRACT: Influenza A virus (IAV) remains a major threat to global health. The emergence of IAV strains resistant to commonly used anti-influenza medicines has made the need for novel broad-spectrum antiviral agents more urgent. The IAV nucleoprotein (NP) is one of the most conserved viral proteins encoded by IAV and is essential for multiple processes in the lifecycle of IAVs. In this study, we screened a high-affinity nanobody, Nb7, which specifically recognizes NP from different subtypes of IAV and broadly inhibits the replication of various IAVs. Mechanistically, Nb7 blocked the nuclear export of viral ribonucleoprotein (vRNP) complexes and the subsequent assembly of progeny virions by specifically binding the nuclear export sequence 1 (NES1) region of the NP. The conserved Q42/E46/K48 residues

SUBMITTER: Shen W 

PROVIDER: S-EPMC12724358 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature