Intranasal bovine/human parainfluenza virus 3 vaccine candidates expressing human metapneumovirus wild-type or pre-fusion F protein elicit protective immunity against human metapneumovirus in hamsters.

ABSTRACT: Human metapneumovirus (HMPV) and human parainfluenza virus 3 (HPIV3) are among the leading causes of severe pediatric respiratory illness worldwide, and there are no licensed vaccines. Here, we describe live-attenuated pediatric intranasal vaccine candidates for dual protection against HMPV and HPIV3. An attenuated chimeric bovine/HPIV3 (B/HPIV3) based on bovine PIV3, with fusion (F) and hemagglutinin-neuraminidase surface glycoproteins replaced by those of HPIV3, was designed to express HMPV subgroup A F protein, the major protective HMPV antigen. Eight viruses were generated, each containing a different version of the HMPV F ORF, including wild-type (wt), codon-optimized (BBopt or GSopt) to increase protein expression, a chimeric form (GSopt-TMCT) with the transmembrane/cytoplasmic tail

SUBMITTER: Saul S 

PROVIDER: S-EPMC12724368 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature