The XRE family protein DbuR is a transcriptional repressor of the <i>dbu</i> operon in <i>Pseudomonas putida</i>.

ABSTRACT: Pseudomonas putida is a ubiquitous, metabolically versatile microbe that has gained visibility as a chassis for bioengineering. The dbu operon in P. putida, comprised of a D-branched-chain amino acid (D-BCAA) oxidase (DbuA), a Rid2 protein (DbuB), and a transporter (DbuC), was previously characterized for its role in the catabolism of D-branched-chain amino acids. In the present study, we show that the addition of any of three D-BCAAs catabolized by these gene products increases expression of the dbu genes. The presence of D-tyrosine does not increase transcription of the dbu genes, nor is it used as a sole nitrogen source by P. putida. Derepression of the dbu operon allows catabolism of D-tyrosine, thus uncovering an additional capacity of

SUBMITTER: Fulton RL 

PROVIDER: S-EPMC12724383 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature