Postnatal Mouse Brain Region-Resolved Peptidomic Resource of Conditional Ndel1 Loss: Comparison of Acidic and Alcoholic Extraction Strategies.

ABSTRACT: The oligopeptidase Ndel1 (NudE neurodevelopment protein 1 like 1) is a multifunctional protein implicated in neurodevelopmental processes, intensively investigated as a potential biomarker in psychiatric disorders. While its roles in regulating the cytoskeleton are well-studied, the global consequences of its loss on the brain's peptide landscape are unknown. This study presents a comprehensive, region-resolved peptidomic resource detailing the consequences of postnatal Ndel1 loss in the mouse cortex, hippocampus, striatum, and cerebellum. We also validated this method of microwave protease inactivation followed by acidic and organic extractions by means of this peptidome analysis across several brain regions. More specifically, using a conditional knockout mouse model with Ndel1 deletion

SUBMITTER: Nani JV 

PROVIDER: S-EPMC12724395 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature