Boxcar Imaging FCS Reveals Membrane Raft Stabilization Kinetics in Antigen-Stimulated Mast Cells.

ABSTRACT: Antigen (Ag) crosslinking of immunoglobulin E-receptor (IgE-FcεRI) complexes in mast cells and consequent coupling with Lyn tyrosine kinase in the plasma membrane inner leaflet stimulates transmembrane signaling to initiate allergic and inflammatory responses. As established previously, this coupling requires formation of liquid-ordered (Lo)-like regions (aka "rafts") around the nano-clustered receptors to facilitate lipid-based partitioning of Lyn via its membrane anchor, followed by receptor phosphorylation mediated by protein-protein interactions. Imaging fluorescence correlation spectroscopy (ImFCS) was previously used to measure diffusion of Lyn-EGFP and its lipid anchor PM-EGFP (both Lo-preferring) as well as EGFP-GG (inner leaflet lipid probe, liquid-disordered (Ld)-preferring) and

SUBMITTER: Yang GS 

PROVIDER: S-EPMC12724450 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature