A validated method for banking mixed glial cultures that yield responsive microglia.

ABSTRACT: Microglia, the primary immune cells of the brain, orchestrate immune responses to both external and internal stimuli in health and disease. Although several cell culture methods exist to model microglia in vitro, isolating loosely adherent microglia from primary murine mixed glial cultures remains valuable for recapitulating in vivo cell states from complex transgenic lines at relatively low cost. However, these methods are constrained by limited temporal control and scalability. To address these challenges, we established a protocol for generating microglia from frozen mixed glial cultures. While freezing introduced measurable differences in microglial morphology and lipid droplet content, microglia derived from frozen cultures responded to environmental stimuli (high glucos

SUBMITTER: Sturno AM 

PROVIDER: S-EPMC12724494 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature