Humanized Extracellular Vesicles for Efficient RNA Delivery.

ABSTRACT: Engineered extracellular vesicles (EVs) are a class of non-viral delivery vectors for RNA-based vaccines and gene therapies. A specialized form of engineered EVs, known as enveloped protein nanocages (EPNs), has been developed to enhance cargo loading and delivery. When EPNs are equipped with a viral fusogen, such as vesicular stomatitis virus glycoprotein (VSV-G), they have been shown to deliver proteins or RNA efficiently into recipient cells. Comparisons across different EPN types and optimization of their different features have been difficult, as assays for their activity have not been reported for single, active units. As we were interested in optimizing EVs, we first developed a biological titration assay inspired by the methods used for infectious viral particles. With this assay,

SUBMITTER: Ma X 

PROVIDER: S-EPMC12724605 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature