Rotational settings quantize nucleosome movement by chromatin regulators.

ABSTRACT: Proper nucleosome positioning is essential for gene regulation and genomic integrity. Regulated nucleosome assembly and positioning results from a need to protect DNA sequences genome-wide, constrained by the known intrinsic sequence preferences of histones. Current models posit that chromatin regulators override the intrinsic preferences to establish the nucleosome landscapes observed in vivo, implying minimal roles for DNA sequence in guiding nucleosomal structure in cells. In contrast, we demonstrate that DNA sequence intrinsically guides the structure and remodeling of nucleosomes from yeast to mammals. We demonstrate that nucleosomes with weak translational settings in vitro, in yeast, and in mammalian cells demonstrate a clear preference for inward-facing A/T dinucleoti

SUBMITTER: La V 

PROVIDER: S-EPMC12724613 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature