TAD boundary architecture and gene activity are uncoupled.

ABSTRACT: Topologically associating domains (TADs) are prominent features of genome organization. A proposed function of TADs is to contribute to gene regulation by promoting chromatin interactions within a TAD and by suppressing interactions between TADs. Here, we directly probe the structure-function relationship of TADs by simultaneously assessing TAD boundary architecture and gene activity at the single-cell and -allele level using high-throughput imaging. We find that while TAD boundaries pair more frequently than non-boundary regions, these interactions are infrequent and are uncorrelated with transcriptional activity of genes within the TAD. Similarly, acute global transcriptional inhibition or gene-specific activation does not alter TAD boundary proximity. Furthermore, disruption of TAD boun

SUBMITTER: Almansour F 

PROVIDER: S-EPMC12724616 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature