Temporal single-cell analysis reveals age-associated delay in immune resolution after respiratory viral infection.

ABSTRACT: Aging is a major risk factor for increased morbidity and mortality following acute respiratory virus infections. To elucidate the immune determinants underlying viral pathogenesis and delayed lung repair in the aged lung, a comprehensive time-course study was conducted. Single-cell RNA sequencing (scRNAseq) and high-dimensional flow cytometry were utilized to compare lungs from young and aged mice infected with influenza A virus (IAV). Aged hosts displayed diminished alveolar macrophage (AM) and dendritic cell (DC) but elevated monocyte-derived macrophage (MoM) and interstitial macrophage (IM) presence following infection. Additionally, enhanced accumulation of adaptive immune cells, including CD4⁺ tissue-resident helper (T_RH) cells, CD8⁺ tissue-resident me

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12724688 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature