A colorimetric method for detecting virulent bacteriophage to Vibrio cholerae in fecal and environmental samples.

ABSTRACT:

Background

Vibrio cholerae are often infected with pathogen-specific virulent bacteriophages (phages) that may be found associated with V. cholerae or independently from the bacteria, especially in environmental water samples. When detected, these phages can serve as a surrogate for detection of V. cholerae and they also have an important role in the ecology of V. cholerae. Vibriophages can be detected using plaque or molecular (PCR) assays, but these methods are time-consuming and require specialized laboratory resources.

Methodology

To increase the accessibility of phage detection for epidemiologic applications, we developed a simple, rapid, and inexpensive colorimetric assay to detect vibriophage that can be scaled quickly to screen large numbers of samples. The assay use

SUBMITTER: Luo W 

PROVIDER: S-EPMC12725621 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature