Functional genomics reveals strain-specific genetic requirements conferring hypoxic growth in <i>Mycobacterium intracellulare</i>.

ABSTRACT: Mycobacterium intracellulare is a major etiological agent of the recently expanding Mycobacterium avium-intracellulare complex pulmonary disease (MAC-PD). Therapeutic regimens that include a combination of macrolides and antituberculous drugs have been implemented with limited success. To identify novel targets for drug development that accommodate the genomic diversity of M. avium-intracellulare, we subjected eight clinical MAC-PD isolates and the type strain ATCC13950 to genome-wide profiling to comprehensively identify universally essential functions by transposon sequencing (TnSeq). Among these strains, we identified 131 shared essential or growth-defect-associated genes by TnSeq. Unlike the type strain, the clinical strains showed increased requirements for genes

SUBMITTER: Tateishi Y 

PROVIDER: S-EPMC12726824 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature