An mTOR-Stat3-Stathmin pathway controls centrosome and microtubule dynamics for oocyte polarization.

ABSTRACT: In animals and plants, egg production is essential for fertility, reproduction, and embryonic development. However, our understanding of the regulatory mechanisms underlying the cellular and developmental programs of oogenesis is lacking. Here, we aimed to identify overlooked regulators of early oogenesis in zebrafish. First, we established a long-term ovary culture system that enables physiological oocyte development from oogonia to primordial follicles, providing an effective ex vivo platform for rapid investigation. Next, we utilized this system for functional screening of candidates from stage-specific oocyte transcriptomic data. We identified mammalian target of rapamycin (mTOR), signal transducer and activator of transcription 3 (Stat3), and the microtubule-destabilizing protein Stat

SUBMITTER: Shawahny A 

PROVIDER: S-EPMC12726958 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature