Staphylococcus aureus causes aberrant epidermal lipid composition and skin barrier dysfunction.

ABSTRACT:

Background

Staphylococcus (S) aureus colonization is known to cause skin barrier disruption in atopic dermatitis (AD) patients. However, it has not been studied how S. aureus induces aberrant epidermal lipid composition and skin barrier dysfunction.

Methods

Skin tape strips (STS) and swabs were obtained from 24 children with AD (6.0 ± 4.4 years) and 16 healthy children (7.0 ± 4.5 years). Lipidomic analysis of STS samples was performed by mass spectrometry. Skin levels of methicillin-sensitive and methicillin-resistant S. aureus (MSSA and MRSA) were evaluated. The effects of MSSA and MRSA were evaluated in primary human keratinocytes (HEKs) and organotypic skin cultures.

Results

AD and organotypic skin colonized with MRSA significantly increased the proportion of lipid

SUBMITTER: Kim J 

PROVIDER: S-EPMC12727050 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature