Unveiling Lipid Droplet Transport Dynamics as Biomarkers of Senescence Using Label-Free, Time-Lapse Holotomography.

ABSTRACT: Accumulation and density of lipid droplets (LDs) in cells have been identified as a potential biomarker to detect senescent cells. However, their intracellular dynamic transport and alterations during senescence remain largely unclear. To address this knowledge gap, senescence was induced in human microglia cells using hydrogen peroxide (H₂O₂) to investigate both short-term and long-term effects of H₂O₂ treatment on LD dynamics. We captured time-lapse holotomograms of LDs using label-free refractive index (RI)-based holotomography and quantified 11 dynamic parameters of LDs through single-particle tracking. These quantified parameters were then compared across healthy cells, short-term H₂O₂-treated pre-senescent cells (H

SUBMITTER: Singam A 

PROVIDER: S-EPMC12727127 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature