Expanding the Eco-collection of Methane-oxidizing Bacteria Inhabiting Rice Roots: Cultivation, Isolation, and Genomic Characterization of Isolates.

ABSTRACT: Flooded rice fields are a major source of atmospheric methane, a strong greenhouse gas second only to carbon dioxide. Rice roots are one of the most important hotspots for methane oxidation in rice fields. However, limited information is available on the physiological and genomic characteristics of methane-oxidizing bacteria (MOB) inhabiting rice roots. In the present study, we isolated MOB from rice roots and characterized the strains phenotypically and genomically. We obtained 100 MOB-enriched cultures from the roots of three rice cultivars (Oryza sativa L. subsp. japonica cv. Nipponbare, O. sativa L. subsp. indica cv. Muha, and Tupa 121-3), in which twelve MOB isolates, two Methylomonas sp., three Methylocystis sp., and seven Methylosinus sp., were successfully purified. They showed dif

SUBMITTER: Oe F 

PROVIDER: S-EPMC12727199 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature