A pseudoenzyme enables indole biosynthesis in eudicot plants.

ABSTRACT: Indole is an important biomolecule in plants, essential for amino acid biosynthesis, defense, pollinator attraction and plant-plant communication. Its biosynthesis is reported to be catalyzed by standalone indole-3-glycerol phosphate lyases, which are, however, absent in core eudicots. Here we show that, in core eudicots, indole production for defense and signaling occurs through an alternative pathway. The tryptophan synthase α subunit (TSA), which is typically complexed with the β subunit (TSB) to synthesize tryptophan through indole as an intermediate, can be hijacked by a noncatalytic paralog of TSB (TSB-like) to produce free indole. TSB-like is a pseudoenzyme that evolved from TSB by mutagenesis of two key essential residues, retaining the ability to allosterically activate TSA to all

SUBMITTER: Florean M 

PROVIDER: S-EPMC12727527 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature