Development of serum glycosylated exosomal microRNAs as biomarkers for early diagnosis of lung adenocarcinoma.

ABSTRACT: Early diagnosis is a major challenge in lung adenocarcinoma (LUAD). Tumor-derived exosomal microRNAs (miRNAs) are promising diagnostic biomarkers, and given the aberrant overexpression of tumor-associated glycans on exosomes, we employed a GlyExo-Capture approach using wheat germ agglutinin (WGA)- and lentil lectin (LCA)-coated magnetic beads to enrich glycosylated exosomes (WGA- and LCA-exosomes) from serum, and then detected exosomal miRNAs in 413 serum samples. Initially, small RNA sequencing was performed on a screening set (n = 30) to obtain candidate WGA-exosomal miRNAs. Moreover, candidate WGA-exosomal miRNAs were identified through RT-qPCR to develop a predictive panel of candidate WGA-exosomal miRNAs combined with candidate LCA-exosomal miRNAs identified in our pilot study

SUBMITTER: Huang Z 

PROVIDER: S-EPMC12727614 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature