A novel peptide-compound conjugate alleviates endotoxin-induced inflammation via NF-κB/MAPK modulation.

ABSTRACT: The anti-inflammatory ability of a novel peptide-compound conjugate, 2IP5MP-CW, synthesized by fusing 2-isopropyl-5-methylphenoxy acetic acid with cysteine and tryptophan, was investigated in this study. 2IP5MP-CW markedly inhibited the growth of Escherichia coli and Staphylococcus aureus and exhibited potent antioxidative activity by suppressing reactive oxygen species production and mitigating oxidative DNA damage induced by lipopolysaccharide (LPS). In RAW 264.7 macrophages, 2IP5MP-CW significantly reduced LPS-induced TNF-α production and suppressed nitric oxide (NO) generation as well as the expression of inflammation-related proteins iNOS and COX-2. The compound also attenuated the phosphorylation of ERK, JNK, NF-κB p65, IKKβ, and IκBα, thereby blocking the nuclear translocation of ph

SUBMITTER: Eom Y 

PROVIDER: S-EPMC12727715 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature