Exosome enrichment from human serum using polyethylene glycol precipitation.

ABSTRACT: Exosomes, nanosized extracellular vesicles that play key roles in intercellular communication, have great potential in diagnostic and therapeutic applications. However, isolating exosomes from complex biological matrices such as human serum remains challenging due to limitations in scalability, cost, and purity of current methods. In this study, we developed and optimized a polyethylene glycol (PEG) precipitation method as a cost-effective alternative to the commercial ExoQuick kit for efficient exosome enrichment from pretreated human serum. The efficiency and reproducibility of PEG precipitation were systematically evaluated and compared with ExoQuick using various analytical techniques, including size exclusion chromatography (SEC), scanning electron microscopy (SEM), nanoparticle track

SUBMITTER: Marzouk RM 

PROVIDER: S-EPMC12727789 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature