M2 macrophage infiltration associated with CXCL8 predicts grade 4 prognosis and differentiates glioma grades.

ABSTRACT:

Background

This study aims to improve glioma-WHO grading by identifying biomarkers distinguishing Gr. 4 from Gr. 3 gliomas and exploring their immune-related prognostic significance.

Methods

We analyzed differentially expressed genes (DEGs) between Gr. 3 and Gr. 4 gliomas using the GSE4290 (31 Gr. 3, 77 Gr. 4) and GSE109857 (34 Gr. 3, 89 Gr. 4) datasets. Key genes were identified through protein-protein interaction (PPI) network and KEGG pathway enrichment analyses. These genes underwent survival analysis and validation using data from The Cancer Genome Atlas (TCGA) and the Chinese Glioma Genome Atlas (CGGA).

Results

Among 23 key genes, CXCL8/IL8 and THBS1 were validated in TCGA and CGGA datasets. CXCL8 significantly correlated with progression-free survival (PFS) in

SUBMITTER: Liu WH 

PROVIDER: S-EPMC12728105 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature