Sympathetic nerve inhibition enhances calvarial bone repair via senescent macrophage-induced osteogenesis and angiogenesis.

ABSTRACT: Bone regeneration is a tightly coordinated process involving multiple cellular and molecular components, with emerging evidence highlighting the pivotal role of the nervous system, especially the sympathetic nervous system, in modulating skeletal repair. However, the mechanistic details of neuro-skeletal interactions during bone healing remain elusive. Here, we inhibited peripheral sympathetic nerves using 6-hydroxydopamine (6-OHDA) in a murine calvarial defect model and performed single-cell RNA sequencing on the injury sites at 7 and 14 days post-injury to delineate the cellular landscape underlying regeneration. Our analyses revealed activation of neurogenesis-associated pathways and dynamic crosstalk between neural and skeletal cells following injury. Sympathetic nerve inhibition signi

SUBMITTER: Zhao L 

PROVIDER: S-EPMC12728170 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature