ABSTRACT: The black soldier fly (BSF), Hermetia illucens, is the insect with the greatest potential to contribute to sustainable human development because of its nutritional characteristics, low environmental impact, and bioremediation ability. In this study, we characterized and quantified the proteome of three BSF stages: egg, larva, and adult, using bottom-up proteomics. A total of 6,116 proteins were identified across all BSF developmental stages. Processes related to information processing, such as chromatin structure, replication, transcription, translation, and the cell cycle, were notably more abundant in eggs than in larvae and adults. Nevertheless, metabolic processes, such as amino acid, carbohydrate, lipid, and nucleotide metabolism, were more abundant in larvae and adults than in eggs.
