Mitochondrial Unfolded Protein Responsive Imaging and Surgical Navigation in Ovarian Cancer.

ABSTRACT: Unfolded proteins, as critical biomarkers in cancer, hold significant potential for tumor-specific imaging. However, the content of unfolded proteins within distinct subcellular organelles varies markedly and reflects divergent physiological implications. Currently, few fluorescent probes enable precise quantification and imaging of mitochondrial unfolded proteins. Herein, we report a fluorescent probe, MAP, for accurate imaging of mitochondrial unfolded proteins. MAP incorporates a triphenylphosphonium group that specifically targets mitochondria, with cellular uptake efficiency proportional to mitochondrial membrane potential. Within mitochondria, the maleimide moiety of MAP covalently reacts with thiol groups on unfolded proteins, restricting molecular rotation and suppressing intramole

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12728763 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature